1- Hormozgan University of Medical Sciences, Bandar Abbas, Iran 2- Tehran Medical Branch, Islamic Azad University, Tehran, Iran 3- Tehran University of Medical Sciences, Tehran, Iran , mansourehsoleimani@gmail.com
Abstract: (23693 Views)
Background : Peripheral blood fibrocytes are a newly identified circulating leukocyte subpopulation that migrates into injured tissue where it may display fibroblast-like properties and participate in wound healing and fibrosis of skin and other organs. In this study, fibrocyte recruitment to skin in a bleomycin-induced dermal fibrosis model of human scleroderma in vivo was studied.
Materials and methods : Sections of skin from normal mice ) control group) and bleomycin-treated C57BL/6 mice (Experimental group) were stained for Procollagen α2 Type I and CD34 and the double stained cells, and also fibrocytes were counted in the tissue sections.
Results : There were more fibrocytes in the dermis of experimental group than control group. There was no significant difference in fibrocyte number between skin samples with 1 week bleomycin treated and 3 weeks treated samples. We also found that fibrocytes (CD34+ and procollagen+) were mainly localized in clusters around blood vessels in the dermis and also individually close to epithelium.
Conclusion : Our data suggest an important role of fibrocytes in the pathology of dermal fibrosis.
Katebi M, Sharifi Z N, Movassaghi S, Chegini H R, Fazelipoor S, Abbasi Moghadam M A et al . Role of fibrocytes (CD34+) derived from peripheral blood in the pathology of dermal fibrosis in a model of scleroderma. MEDICAL SCIENCES 2011; 21 (2) :97-100 URL: http://tmuj.iautmu.ac.ir/article-1-449-en.html