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The effect of silver nanoparticles synthesized with ginger on reducing vanA gene expression in vancomycin-resistant Enterococcus faecalis strains
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Ehsan Al-Sabrawi1    , Farahnaz Molavi 2 |
1- MSc Student in Cellular and Molecular Sciences, Islamic Azad University, Mashhad Branch, Department of Biology, Mashhad, Iran
2- Islamic Azad University, Mashhad Branch, Department of Biology, Mashhad, Iran , FARAHMOLAVI@GMAIL.COM |
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Abstract: (162 Views) |
Background: One of the factors causing the resistance of Enterocus faecalis bacteria to the vancomycin antibiotic is the vanA gene, and the purpose of this research is to investigate the effect of silver nanoparticles on the expression of the vanC resistance gene.
Materials and methods: In this study, 98 isolates were examined after confirming the presence of the vanA gene and the antibiogram test of the treatment, the bacteria with multidrug resistance with the lowest ability to silver nanoparticles were performed and with the Real-time PCR technique, the data were analyzed. It was related to the change of gene expression. vanA was transformed in two analysis groups.
Results: Antibiotic resistance of the strains was 50% to vancomycin, 32% to ciprofloxacin, 15% to chloramphenicol, 5% to teicoplanin, 43% to erythromycin, 79% to amikacin, 80% to tetracycline, 80% to cephalexin, 85% to penicillin, and 1% to linozolid. Real-time analysis on the resistant strains in which the presence of vanA gene was confirmed by PCR and treated with silver nanoparticles showed that the effect of nanoparticles on the expression of vanA gene is significant.
Conclusion: Existing 87% of the multidrug resistance in 64 confirmed strains indicates that the issue of multidrug resistance is a serious warning in the use of antibiotics to treat infections caused by Enterococcus faecalis and the effectiveness of silver nanoparticles on vanA gene expression indicates that It can be used as an alternative to antibiotics in infections caused by Enterococcus faecalis.
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| Keywords: Streptococcus, Vancomycin, Metal nanoparticles, Multidrug-resistant, Ginger |
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Full-Text [PDF 642 kb]
(89 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Molecular Biology
Received: 2023/05/30 | Accepted: 2023/08/29 | Published: 2024/02/29
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