@ARTICLE{Harzandi, author = {Torfeh, Zahra and Harzandi, Naser and Ghaderi, Mostafa and }, title = {Detection of Human Group A Rotavirus in Urban Sewage, Hospital Sewage and River Water Samples in Alborz Province Using ELISA Method}, volume = {28}, number = {2}, abstract ={Background: Human group A rotaviruses are the most common cause of severe diarrhea among children <5 years of age worldwide. Rotaviruses are widely present in environmental water. The aim of this study was to detect the human group A rotaviruses in urban sewage, hospital sewage and river water samples in Alborz province using ELISA method. Materials and methods: This cross-sectional study was carried out on 76 samples collected from both influent and effluent water of 4 sewage treatment systems, hospital sewage, Karaj and Baraghan rivers in Alborz province. All samples were concentrated using pellet method. Afterwards, human group A rotaviruses were detected using ELISA method. Results: In total, rotaviruses were identified in 6 samples (7.89%) using ELISA method. Three positive samples (50%) were related to the raw sewage influent and three positive samples (50%) were related to the Karaj river. The frequency of rotavirus detection in summer, autumn and winter was 1 sample (16.66%), 3 samples (50%) and 2 samples (33.33%), respectively. Conclusion: This study showed the contamination of environmental water by human group A rotaviruses. Therefore, it is necessary to constantly monitor sewage treatment systems and river water for detecting rotaviruses. Keywords: Human group A rotaviruses, Environmental water, Pellet method, ELISA. }, URL = {http://tmuj.iautmu.ac.ir/article-1-1404-en.html}, eprint = {http://tmuj.iautmu.ac.ir/article-1-1404-en.pdf}, journal = {Medical Sciences Journal of Islamic Azad University}, doi = {10.29252/iau.28.2.124}, year = {2018} }