RT - Journal Article T1 - Frequency and importance of jak2v617f mutation among Iranian patients with polycythemia vera, essential thrombocythemia and primary myelofibrosis JF - iau-tmuj YR - 2010 JO - iau-tmuj VO - 20 IS - 1 UR - http://tmuj.iautmu.ac.ir/article-1-280-en.html SP - 0 EP - 0 K1 - Myeloproliferative neoplasms K1 - JAK2-V617F mutation K1 - Allele- specific PCR AB - Background: Detection of JAK2V617F mutation was widely used in the diagnosis and classification of myeloproliferative neoplasms. In this study, frequency of JAK2V617F mutation among Iranian patients with polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF) was studied. Materials and methods: In this basic study, blood samples of 174 patients with polycythemia vera (n= 57), essential thrombocythemia (n= 84) and primary myelofibrosis (n= 33) were evaluated for JAK2V617F mutation. Genomic DNA was extracted from peripheral blood. After quality control of extracted DNA, the JAK2–V617F mutation was analyzed using allele-specific PCR. All PCR products were analyzed by polyacrylamide gel (5%) electrophoresis and silver staining. Results: One hundred and eleven out of 174 patients (63.8%) were positive for the presence of the JAK2V617F mutation. Frequency of mutation was 82% (47/57) in PV, 57% (48/84) in ET and 48% (16/33) in PMF. Conclusion: This study showed that detection of JAK2-V617F mutation using allele-specific PCR lead to better diagnosis and treatment of Iranian patients with different MPNs. LA eng UL http://tmuj.iautmu.ac.ir/article-1-280-en.html M3 ER -