TY - JOUR T1 - Assessment of antimutagenicity and anticancer effects of Citrus Limon TT - بررسی خواص ضدجهشی و ضدسرطانی لیموشیرین Citrus Limon JF - iau-tmuj JO - iau-tmuj VL - 18 IS - 2 UR - http://tmuj.iautmu.ac.ir/article-1-141-en.html Y1 - 2008 SP - 91 EP - 96 KW - Antimutagenicity KW - Anticancer KW - Citrus Limon KW - Human Astrocytoma cancer cells KW - Ames Test N2 - Background: Currently cancer is considered as one of the main causes of mortality globally. Many chemicals in our environment can cause genetic mutations and are potentially responsible for millions of cancer-related deaths. The scientists are nowadays looking for food materials which can potentially prevent cancer occurrence. The aim of this research was to examine antimutagenicity and anticancer effects of Citrus Limon fruit juices.Material and methods: In the present study Human Astrocytoma cancer cells were cultured in DMEM (Gibco), supplemented with 10% fetal calf serum, penicillin, streptomycin, L-glutamine and incubated at 37 ºC for 2 days. In addition, cancer cell lines were treated by Citrus Limon fruit juice and cellular vital capacity was determined by MTT. The Citrus Limon fruit juice was subsequently evaluated in terms of antimutagenicity and anticancer properties by a standard reverse mutation assay (Ames Test). For performing Ames Test salmonella typhimurium TA100 strain was chosen based on the fact that it had a specific mutation in the histidine operon, requiring histidine from a foreign source to ensure its growth. The mentioned strain gives rise to reverted colonies exposing to carcinogen substance (Sodium Azide). Results: During MTT, human Astrocytoma cell line revealed to have a significant cell death when compared with controls (p M3 ER -