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Association of D19H polymorphism of ABCG8 gene with gallstone susceptibility
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Saman Milanizadeh1   , Amir Houshang Mohammad Alizadeh2 , Pedram Azimzadeh1 , Sara Romani3 , Mohammad Roshani4 , Mahsa Khan Yaghma5 , Seyed Reza Mohebbi 6, Mohammad Reza Zali2 |
1- MSc of Cellular and Molecular Biology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Gastroenterologist, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- MSc of Virology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Gastroenterologist, Kurdistan University of Medical Sciences, Sanandaj, Iran
5- MSc of Biology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6- PhD of Virology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , srmohebbi@gmail.com |
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Abstract: (10692 Views) |
Background: Increased level of cholesterol may be saturated in the gall bladder resulting in gall stone. Product of ABCG8 gene is responsible for evacuation of cholesterol from cells and made dissolved cholesterol in bile. It appears that genetic changes of this gene can alter its function and make gall stone. This study was designed to evaluate association of D19H polymorphism of ABCG8 gene with gallstone susceptibility.
Materials and Methods: This was a case-control research that was conducted on 100 gallstone patients and 106 healthy controls without any family history of gallstone. For DNA extraction, salting out method has been used and for detection of alleles in polymorphic region, PCR-RFLP has been used.
Results: heterozygote genotype in this polymorphic region (G/C) had association with gallstone formation (OR=7.14 95%CI=3.47-14.66 P<0.001). We found that people with heterozygote genotype had more than 7 times susceptibility to gall stone compared to healthy controls.
Conclusion: This study confirms previous studies about D19H polymorphism on ABCG8 gene and shows importance of this SNP in different populations. For this reason, we can use analysis of this region to predict susceptibility to gall stone formation. |
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| Keywords: Gall stone, ABCG8, polymorphism, D19H |
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Full-Text [PDF 225 kb]
(2836 Downloads)
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Semi-pilot: case-control |
Subject:
Molecular Biology
Received: 2013/09/22 | Published: 2013/09/15
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