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Evaluation of in vitro quality of none agitated platelet concentrate
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Hossein Taymory Naghadeh1   , Mohammad Fallahtafti 2, Seyed Mohammad Masoud Shoshtarian3 |
1- Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, IBTO, Tehran, Iran
2- Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, IBTO, Tehran, Iran , m.falah@ibto.com
3- Department of Biophysic, Islamic Azad University, Tehran Medical Branch, Tehran, Iran |
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Abstract: (10638 Views) |
Background: Platelet concentrate (PC), in the different times from collection until transfusion, undergo a considerable time period without agitation. The aim of this study was to compare the continuously agitated PCs in vitro properties, with non agitated PCs for 6 hours, just after termination of interruption in day3 of storage in Iranian blood transfusion organization (IBTO), Tehran.
Materials and Methods: 20 PCs were obtained from platelets pooled and divided into two groups. The first group designated as reference group with continuously agitating PCs the second group or test group stored at stationary condition for 6 hrs under the same environment in the incubator. Day three was selected for evaluation, because PCs are transported to hospitals on the beginning of day three, the time that the agitation is interrupted. In-vitro variables, including pH, swirling, Ristocetin-induced aggregation, LDH (viability), PF4 release and P- selectin expression (activation markers) were measured.
Results: The mean level of pH in agitated and non agitated groups were 7.16±0.08 and 7.22±0.16, respectively (p=0.3). Ristocetin-induced aggregation, LDH and swirling studies showed no significant differences between two groups, but 6h PCs showed less PFA release (p=0.015) and P-selectin expression (p=0.006) which was the important achievement.
Conclusion: According to findings of this study, especially for pH and swirling, 6hrs non agitated PC’s at 22-24˚С has good quality compared with routine PCs. |
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| Keywords: Random PCs, Continuous agitation, Interruption agitation |
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Full-Text [PDF 1053 kb]
(3981 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Oncology
Received: 2013/06/19 | Published: 2013/06/15
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