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:: Volume 22, Issue 3 (Fall 2012) ::
MEDICAL SCIENCES 2012, 22(3): 175-183 Back to browse issues page
Growth inhibitory and differentiating effects of ethyl acetate soluble metabolite of Iranian native bacteria, Streptomyces calvus, in human myeloid leukemia K562 cell line
Seyed Mohammad Amin Moosavi * 1, Farideh Ghanbarvand 2, Alireza Dehnad 3
1- Department of Zoology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran , moosav_m@ tabrizu.ac.ir
2- MSc Student, Department of Zoology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
3- Regional Agriculture Biotechnology, Research Institute of West and Northwest, Tabriz, Iran
Abstract:   (13731 Views)
Background: Because drug resistance is one of the most important problems in patients with chronic myeloid leukemia (CML), finding new anti-cancer drugs especially from natural sources is research priority. Therefore, in this study, anti-cancer effects of ethyl acetate soluble metabolite of Iranian native bacteria, Streptomyces calvus, were studied using human chronic myeloid leukemia K562 cells.
Materials and methods: In this experimental trail, ethyl acetate soluble metabolites were isolated from S. calvus bacteria. K562 cell line was treated by various concentrations of this metabolite for 12- 72 h intervals. Anti-proliferative effects of ethyl acetate soluble metabolite were studied by trypan blue exclusion test. Wright-Giemsa staining and latex particle phagocytosis assay were used to study differentiated cells.
Results: Ethyl acetate soluble metabolite induced growth inhibition in K562 cells in concentration and time- dependent manner. At the concentration of 200 ng/ml, the growth was inhibited 19-50% after 12-72h. Latex particle phagocytosis assay and Wright- Giemsa staining results revealed that K562 cells were differentiated toward monocytic- macrophagic lineage.
Conclusion: According to growth inhibitory and differentiating effects of S.calvus metabolite in K562 cells, this metabolite can be proposed for more investigations in differentiation therapy of CML patients.
Keywords: Chronic myeloid leukemia, Differentiation, Natural products, Streptomyces
Full-Text [PDF 309 kb]   (1454 Downloads)    
Type of Study: Experimental | Subject: Animal Biology
Received: 2012/09/23
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Moosavi S M A, Ghanbarvand F, Dehnad A. Growth inhibitory and differentiating effects of ethyl acetate soluble metabolite of Iranian native bacteria, Streptomyces calvus, in human myeloid leukemia K562 cell line . MEDICAL SCIENCES. 2012; 22 (3) :175-183
URL: http://iau-tmuj.ir/article-1-589-en.html


Volume 22, Issue 3 (Fall 2012) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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