|
The regenerative effects of Thymus vulgaris extract on beta cells of pancreas of streptozotocin induced diabetic Wistar rats
|
Parichehre Yaghmaie   1, Esfandiar Heydarian2 , Nazanin Poorbahman3 |
1- Islamic Azad University, Science and Research Campus, Tehran, Iran , yaghmaei_p@yahoo.com
2- Shahrekord University of Medical Sciences, Shahrekord, Iran
3- Islamic Azad University, Science and Research Campus, Tehran, Iran |
|
|
Abstract: (27952 Views) |
Background: Diabetes is the commonest carbohydrate metabolism disease which may be either due to insulin secretion deficiency or insulin dysfunction or both. This study was conducted to evaluate hypoglycemic effects of Thymus vulgaris extract and its regenerative effects on beta cells of pancreas.
Materials and methods: In this study, male Wistar rats weighting 200-250 grams were included and maintained at 22±2 degrees Celsius. The rats were divided into 5 groups of 6 rats each. Experimental groups were made diabetes by streptozotocin (STZ) (70 mg/kg i.p.). Three experimental groups were gavaged with extract (100, 200 and 400 mg/kg) for 21consecutive days. After the end of treatment, blood samples were drown from heart and serum glucose, liver glycogen, SOD enzyme malondialdehyde (MDA) level, plasma antioxidant power were measured. Finally, the rats were deeply anesthetized and sacrificed for histological evaluation of their pancreas.
Results: Serum levels of glucose and MDA were increased (p<0.001) and serum SOD activity and liver glycogen were decreased (p<0.001) significantly in the diabetic groups as compared with the control group. In diabetic rats treated with Thymus vulgaris extract, serum SOD activity, plasma antioxidant power and liver glycogen were increased and serum MDA levels and serum glucose were decreased (p<0.001). Thymus vulgaris extract increased the size and the number of pancreatic Langerhans islets looking like of normal rats.
Conclusion: Treatment of diabetic rats with Thymus vulgaris extract not only decreases the level of serum glucose, but also has beneficial regenerative effect on Langerhans islands. |
|
| Keywords: Thymus vulgaris, Pancreas, Diabetic mellitus, Rat |
|
|
Full-Text [PDF 137 kb]
(3005 Downloads)
|
Semi-pilot: Experimental |
Subject:
Animal Biology
Received: 2011/11/27 | Accepted: 2013/06/30 | Published: 2013/06/30
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
