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Evaluation of induced apoptosis of apigenin in 3 lines of human B cell lymphoma in vitro
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Mehrdad Hashemi   1, Mehdi Nouri Lang , Maliheh Entezari , Shohreh Nafisi , Hossein Nowrouzi |
| 1- , hashemi@iautmu.ac.ir |
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Abstract: (27839 Views) |
Background: Apoptosis or programmed cell death (PCD) is an important mechanism in both development and homeostasis of human tissues for the removal of superfluous cells and its induction is an effective method in the treatment of cancer. The aim of this study was the evaluation of induced apoptosis of apigenin in the human B cell lymphoma.
Materials and methods: In this experimental study, three human lymphoma B cells were cultured in RPMI1610, supplemented with 10% fetal calf serum, peniciline-streptomycin and L-glutamine, at 37ºC for 2 days. Cancer cell lines were treated by apigenin and Cellular vital capacity was determined by MTT. Then, effects of apigenen on human lymphoma B cells were examined by flowcytometry technique.
Results: During MTT, human lymphoma B cell line revealed significant apoptosis at 10, 15 and 20 μg/ml concentrations compared with controls (p<0.01). Flowcytometry assay showed that apoptotic bodies were significantly different in three human lymphoma B cells and 48 hours was appropriate time for inducing apoptosis.
Conclusion: This study revealed the anticancer effects of apigenin and its effecst on apoptosis on human lymphoma B cells in vitro. |
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| Keywords: Apoptosis, Apigenin, Human lymphoma B cells |
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Full-Text [PDF 239 kb]
(3709 Downloads)
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Subject:
Epidemiology
Received: 2006/09/6 | Published: 2009/07/15
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