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:: Volume 18, Issue 4 (Winter 2008) ::
MEDICAL SCIENCES 2008, 18(4): 209-215 Back to browse issues page
Effect of Dibenzo-18-crown-6 on haematopoitic cells colony formation of mouse bone marrow
Masoud Mashhadi Akbar Boojar * 1, Kazem Parivar , Abbas Shokravi , Maral Molaie
1- , aboojar@yahoo.com
Abstract:   (19442 Views)
Background: Known biological effects of crown ethers influence us to investigate its effect in human body and use in drug production. The aim of this study was to evaluate the effects of Dibenzo-18-crown-6 on colony formation of bone marrow cells of mouse in culture medium and to find the probable mechanisms of the process.
Materials and Methods: In this experimental study, red bone marrow cells of mouse (Balb/C) were used as source of haemapoietic stem cells. Culture of red bone marrow cells was done in the form of semi solid in the presence of different concentrations of crown ether, cAMP, PHO4H2K and PO4H2Na.
Results: Different concentrations of crown ether have significant inhibitory effects on colonizing of red bone marrow cells. The maximum inhibitory effect was observed in concentration of 80 mg/ml crown ether and no colony was found. The inhibitory effects of crown ether was diminished in the presence of cAMP with concentration of 10× 10-6 mol , PO4H2Na with concentration of 10× 10-6 mol and PO4H2K with concentration of 5×106 mol, but it couldn’t achieve the level of control group without presence of crown ether. HPLC method demonstrated that remaining level of crown ether reached to zero after sixth hour.
Conclusion: The inhibitory effects of crown ether in control of colonizing the red bone marrow cells occur via decreasing of cations value and changing the physiological function of membrane resulting disorder in the cAMP signaling pathway.
Keywords: Dibenzo- 18- crown-6, red bone marrow, Colony formation
Full-Text [PDF 193 kb]   (1268 Downloads)    
Subject: Epidemiology
Received: 2006/09/6
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Mashhadi Akbar Boojar M, Parivar K, Shokravi A, Molaie M. Effect of Dibenzo-18-crown-6 on haematopoitic cells colony formation of mouse bone marrow . MEDICAL SCIENCES. 2008; 18 (4) :209-215
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Volume 18, Issue 4 (Winter 2008) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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