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:: Volume 28, Issue 3 (spring 2018) ::
MEDICAL SCIENCES 2018, 28(3): 212-219 Back to browse issues page
The pattern of antibiotic resistance within clinical isolates of Pseudomonas aeruginosa and detection of AmpC
Mahsa Jamshidi Gohar 1, Nahid Rahimi Fard 2, Seyed Reza Hosseini Doust * 3
1- MSc student in department of Microbiology, Medical Sciences University Azad Islamic, Tehran/Iran
2- Iranian Food and drug Administration, Ministry of Health and Medical Education
3- in Department of Microbiology, Tehran Medical Sciences Islamic Azad University, Tehran/Iran , rhdoust@iaups.ac.ir
Abstract:   (158 Views)
Background: Despite the improvements in hospital care and the introduction of a wide range of antimicrobial agents, Pseudomonas aeruginosa is also a common cause of infection in patients hospitalized in different wards of the hospital. Due to the rising resistance of this bacterium to antibacterial drugs, the importance of its resistance is increased. The enzyme β-lactamase AMP-C is a type of cephalosporinase coded on the chromosome of the bacterium. In many bacteria, induction of AMP-C enzymes can occur at high levels by many mutations. In this paper, the detection of AMP-C gene was reported in clinical isolates of Pseudomonas aeruginosa.
Materials and methods: 80 Pseudomonas aeruginosa bacteria, verified by gram stain and biochemical tests, were isolated from wound samples collected from patients with burning at burning hospital in Tehran. For identifying antibiotic resistance, in vitro susceptibility of 80 Pseudomonas aeruginosa isolates to 15 antimicrobial agents, includimg colistin, amoxicillin, ceftriaxone, cefixime, cefalotin, ciprofloxacin, amikacin, doxycyclin, ampicillin, trimethoprim, ceftazidime, gentamicin, cefotaxime, piperacilin and imipenem was performed by Kirby-Bauer’s disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI, 2016) guideline. PCR method was used to identify AMP-C gene in 80 Pseudomonas aeruginosa strains.
Results: Pseudomonas aeruginosa strains showed the highest resistance to cephalotin (100%), doxycycline (100%), cefixime (100%), amoxicillin (100%), ampicillin (100%), amikacin (100%) and teri-methaprime (100%). Highest sensitivity of Pseudomonas aeruginosa strains was observed to colistin (67.5%), gentamicin (32.5%), piperacillin (30%), ciprofloxacin (28.7%), imipenem (18.7%), ceftazidime (13.7%), ceftriaxone (11.2%) and cefotaxime (10%). AMP-C gene was detected in 5% of isolates.
Conclusion: According to the results of this study, in many Pseudomonas aeruginosa strains, the presence of all resistance genes in an isolate was not observed. Therefore, it can be hoped that β-lactamase enzymes are not coded in most isolates.
 
Keywords: AMP-C, Antibiotics resistance, P. aeruginosa, Clinical isolates.
Keywords: AMP-C, Antibiotics resistance, P. aeruginosa, Clinical isolates.
Full-Text [PDF 410 kb]   (28 Downloads)    
Type of Study: Experimental | Subject: Microbiology
Received: 2018/02/14 | Accepted: 2018/05/7 | Published: 2018/09/22
ENG-PDF [PDF 720 KB]  (5 Download)
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Jamshidi Gohar M, Rahimi Fard N, Hosseini Doust S R. The pattern of antibiotic resistance within clinical isolates of Pseudomonas aeruginosa and detection of AmpC . MEDICAL SCIENCES. 2018; 28 (3) :212-219
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Volume 28, Issue 3 (spring 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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