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:: Volume 28, Issue 2 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(2): 124-129 Back to browse issues page
Detection of Human Group A Rotavirus in Urban Sewage, Hospital Sewage and River Water Samples in Alborz Province Using ELISA Method
Zahra Torfeh 1, Naser Harzandi * 2, Mostafa Ghaderi 3
1- M.Sc. Student of Microbiology, Department of Microbiology, Faculty of Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran
2- Assistant Professor Microbiology, Department of Microbiology, Faculty of Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran , naser.harzandi@kiau.ac.ir
3- Assistant Professor virology, Department of Microbiology, Faculty of Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract:   (284 Views)
Background: Human group A rotaviruses are the most common cause of severe diarrhea among children <5 years of age worldwide. Rotaviruses are widely present in environmental water. The aim of this study was to detect the human group A rotaviruses in urban sewage, hospital sewage and river water samples in Alborz province using ELISA method.
Materials and methods: This cross-sectional study was carried out on 76 samples collected from both influent and effluent water of 4 sewage treatment systems, hospital sewage, Karaj and Baraghan rivers in Alborz province. All samples were concentrated using pellet method. Afterwards, human group A rotaviruses were detected using ELISA method.
Results:  In total, rotaviruses were identified in 6 samples (7.89%) using ELISA method. Three positive samples (50%) were related to the raw sewage influent and three positive samples (50%) were related to the Karaj river. The frequency of rotavirus detection in summer, autumn and winter was 1 sample (16.66%), 3 samples (50%) and 2 samples (33.33%), respectively.
Conclusion: This study showed the contamination of environmental water by human group A rotaviruses. Therefore, it is necessary to constantly monitor sewage treatment systems and river water for detecting rotaviruses.
Keywords: Human group A rotaviruses, Environmental water, Pellet method, ELISA.
 
Keywords: Human group A rotaviruses, Environmental water, Pellet method, ELISA.
Full-Text [PDF 431 kb]   (79 Downloads)    
Type of Study: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2017/09/23 | Accepted: 2017/11/22 | Published: 2018/06/19
References
1. Ramirez-Castillo FY, Loera-Muro A, Jacques M, Garneau P, Avelar-Gonzalez FJ, Harel J, et al. Waterborne pathogens: detection methods and challenges. Pathogens 2015;4:307-34. [DOI:10.3390/pathogens4020307]
2. Kargar M, Javdani N, Najafi A, Tahamtan Y. First molecular detection of group A rotavirus in urban and hospital sewage systems by nested-RT PCR in shiraz, Iran. J Environ Health Sci Eng 2013;11:2-6. [DOI:10.1186/2052-336X-11-4]
3. Harzandi N, Samiee P, Dezfulian M. Molecular detection and genotyping of rotaviruses in children with acute gastroenteritis in Karaj hospital. J Qazvin Univ Med Sci 2014;18:21-7.
4. Kiulia MN, Nynke H, Vermeulen LC, Obara MA, Medema G, Rose JB. Global occurrence and emission of rotavirus to surface waters. Pathogens 2015;4:229-55. [DOI:10.3390/pathogens4020229]
5. Carrol KC, Hobden JA, Miller S, Morse SA, Mietzner TA, Detrick B, et al. Jawetz-Melnick, & Adelberg,s Medical Microbiology. 27th ed. New York: McGraw Hill; 2016. P. 531-6.
6. Koroglu M, Yakupogullari Y, Otlu B, Ozturk S, Ozden M, Ozer A, et al. A waterborne outbreak of epidemic diarrhea due to group A rotavirus in Malatya, Turkey. New Microbiol 2011;34:17-24.
7. Ruggeri MF, Bonomo P, Ianiro J, Battistone A, Delogu R, Germinario C, et al. Rotavirus genotypes in sewage treatment plants and in children hospitalized with acute diarrhea in Italy in 2010 and 2011. Appl Environ Microbiol 2015;81:241-9. [DOI:10.1128/AEM.02695-14]
8. Kargar M, Najafi A, Zandi K, Barazesh A. Frequency and demographic study of rotavirus acute gastroenteritis in hospital children of Borazjan city during 2008-2009. JSSU 2011;19:94-103.
9. Kargar M, Razeghi Z, Najafi A. Removal of human rotaviruses type A from concentrated sewage of Ghods and Mahallati wastewater treatmen systems in Tehran. J Health 2016;7:395-403.
10. Javdani N, Kargar M, Ghodsi M. The assessment of efficiency of eliminating group A human rotaviruses in urban and hospitalized sewage refining system of Shiraz city. Medical Sciences 2012;22:226-31.
11. Bitton G. Wastewater Microbiology. 4th ed. United States of America: A John Wiley & Sons; 2011. P. 151-2.
12. Rodriguez-Diaz J, Querales L, Caraballo L, Vizzi E, Liprandi F, Takiff H, et al. Detection and characterization of waterborne gastroenteritis viruses in urban sewage and sewage-polluted river waters in Caracas, Venezuela. Appl environ microbiol 2009;75:387-94. [DOI:10.1128/AEM.02045-08]
13. Miagostovich MP, Ferreira FFM, Guimarães FR, Fumian TM, Mendes LD, Luiz B Luz S, et al. Molecular detection and characterization of gastroenteritis viruses occurring naturally in the stream waters of Manaus, Central Amazônia, Brazil. Appl environ microbiol 2008;47:375-82. [DOI:10.1128/AEM.00944-07]
14. Kittigul L, Raengsakulrach B, Siritantikorn S, anyok R, Utrarachkij F, Diraphat P, et al. Detection of Poliovirus, Hepatitis A Virus and rotavirus from sewage and water samples. Southern Asian J Trop Med Public Health 2000;31:41-6.
15. Hovi T, Stenvik M, Partanen H, Kangas A. Poliovirus surveillance by examining sewage specimens. Quantitative recovery of virus after introduction into sewerage at remote upstream location. Epidemiol infect 2001;127:101-6. [DOI:10.1017/S0950268801005787]
16. Kargar M, Sadeghpour S, Nategh. Environmental surveillance of non-polio enterovirus in Iran. Virol J 2009;6:1-5. [DOI:10.1186/1743-422X-6-149]
17. Chigor VN, Okoh AI. Quantitative RT-PCR Detection of Hepatitis A Virus, Rotaviruses and Enteroviruses in the Buffalo River and Source Water Dam in the Eastern Cape Province of South Africa. Int J Environ Res Public Health 2012;9:4017-32. [DOI:10.3390/ijerph9114017]
18. Rigotto C, Victoria M, Moresco V, Kolensnikovas CK, Correa AA, Souza DSM, et al. Assessment of adenovirus, hepatitis A virus and rotavirus presence in environmental samples in Florianopolis, South Brazil. J Appl Microbiol 2010;109:1979-87. [DOI:10.1111/j.1365-2672.2010.04827.x]
19. Sdiri-Loulizi K, Hassine M, Aouni Z, Gharbi-Khelifi H, Chouchane S, Sakly N, et al. Detection and molecular characterization of enteric viruses in environmental samples in Monastir, Tunisia between January 2003 and April 2007. J Appl Microbiol 2010;109:1091-104. [DOI:10.1111/j.1365-2672.2010.04772.x]
20. Li D, Gu AZ, Zeng S, Yang W, He M, Shi H. Evaluation of the infectivity, gene and antigenicity persistence of rotaviruses by free chlorine disinfection. J Environ Sci (China) 2011;23:1691-8. [DOI:10.1016/S1001-0742(10)60623-7]
21. Xue B, Li C, Zhang B, Zhao T, Shen Z, Qiu Z, et al. Quantitative reverse transcription PCR to determine the inactivation of Human Rotavirus by chlorine. Int J Hyg Environ Health 2017;220:719-25. [DOI:10.1016/j.ijheh.2017.02.007]
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Torfeh Z, Harzandi N, Ghaderi M. Detection of Human Group A Rotavirus in Urban Sewage, Hospital Sewage and River Water Samples in Alborz Province Using ELISA Method. MEDICAL SCIENCES. 2018; 28 (2) :124-129
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Volume 28, Issue 2 (winter 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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