:: Volume 28, Issue 2 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(2): 104-116 Back to browse issues page
Role of extracted pigment from environmental isolated bacteria on antibiotic resistant isolated microorganisms from diabetic patients
Noushin Nahid1 , Nima Bahador 2, Nematollah Razmi3
1- MSc Student of Microbiology, Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Fars, Iran; Department of Microbiology, College of Science, Agriculture and Modern technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Microbiology, Department of Microbiology, College of Science, Agriculture and Modern technology Shiraz branch, Islamic Azad University, Shiraz, Iran , bahador@iaushiraz.ac.ir
3- Microbiology, Department of Microbiology, College of Science, Agriculture and Modern technology Shiraz branch, Islamic Azad University, Shiraz, Iran
Abstract:   (4042 Views)
Background: Diabetes is one of the most important metabolic diseases in the world that can ultimately lead to ulceration and amputation of lower limbs. The aim of this study was pigment extraction from isolated environmental bacteria and their effect on antibiotic resistant bacteria cooperated on infected diabetic foot.
Materials and methods: In this project, dominant bacteria were identified using biochemical tests and antibiotic susceptibility was evaluated. Then, effect of different solvent was evaluated on the extraction of pigments using thin layer chromatography. Finally the affected bacteria were molecularly identified.
Results: The results obtained from this study indicated that the most common isolated bacteria were Escherichia coli and Staphylococcus aureus. The results from antibiogram tests indicated that most of the gram positive isolates had same results and the gram negative was sensitive to Meropenem and Imipenem. Among extracted pigments, 4 different pigment which is effective on antibiotic resistant bacteria according to molecular technique were Brachybacterium sp. IARI-ABL-35, Brachybacterium nesterenkovii strain DSM 9573, Brevundimonas sp. NeomS2D Kocuria sp. YIM 75764.
Conclusion: According to biologic properties of bacterial pigments, we could use them in the treatment of various diseases.
Keywords: Antibiotic susceptibility, Bacterial infection, Bacterial pigment, Diabetes mellitus, Thin-layer chromatography.
Full-Text [PDF 736 kb]   (3339 Downloads)    
Semi-pilot: Experimental | Subject: Microbiology
Received: 2017/05/17 | Accepted: 2017/09/3 | Published: 2018/06/19
References
1. Bakker K, Apelqvist J, Lipsky BA, Van Netten JJ; International Working Group on the Diabetic Foot. The 2015 IWGDF guidance documents on prevention and management of foot problems in diabetes: development of an evidence‐based global consensus. Diabetes Metab Res Rev 2016;1:2-6. [DOI:10.1002/dmrr.2694]
2. Federation ID. IDF diabetes atlas. Brussels: International Diabetes Federation. 2013.
3. Lipsky BA, Peters EJ, Berendt AR, Senneville E, Bakker K, Embil JM, et al. Specific guidelines for the treatment of diabetic foot infections 2011. Diabetes Metab Res Rev 2012;1:234-5. [DOI:10.1002/dmrr.2251]
4. Gardner SE, Frantz RA. Wound bioburden and infection-related complications in diabetic foot ulcers. Biol Res Nurs 2008;10:44-53. [DOI:10.1177/1099800408319056]
5. Zanella MC, Kressmann B, Wuarin L, Coulin B, Maître S, Suva D, et al. Microbiology and antibiotic treatment of diabetic foot infection. Rev Med Suisse 2016;12:732-7.
6. Pednekar S, Pol SS, Kamble SS, Deshpande SK, Bharadwag r. Drug resistant anaerobic infections: are they complicating diabetic foot ulcer. Int J Healthc Biom Res 2015;3:142-8.
7. Rashid M, Fakruddin M, Mazumdar RM, Kaniz F, Chowdhury M. Anti-Bacterial Activity of Pigments Isolated From Pigment-Forming Soil Bacteria. Br J Pharm Res 2014;4:880-894. [DOI:10.9734/BJPR/2014/5148]
8. Giri AV, Anandkumar N, Muthukumaran G, Pennathur G. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol 2004;4:11. [DOI:10.1186/1471-2180-4-11]
9. Ahmad WA, Ahmad WY, Zakaria ZA, Yusof NZ. Isolation of Pigment-Producing Bacteria and Characterization of the Extracted Pigments.Application of Bacterial Pigments as Colorant 2012;2:25-44. [DOI:10.1007/978-3-642-24520-6_2]
10. Shatila F, Yusef H, Holail H. Pigment production by Exiguobacterium aurantiacum FH, a novel Lebanese strain. Int J Curr Microbiol App Sci 2013;2:176-91.
11. Goswami B, Bhowal J. Identification and characterization of extracellular red pigment producing bacteria isolated from soil. Int J Curr Microbiol App Sci 2014;3:169-76.
12. Ahmadi Fakhr F, Khanafari A. Marandi R. Determination of optimum conditions for the production of pigment Prodigiosin from dissected plants, Fourth National Biotechnology Conference of the Islamic Republic of Iran. 2005;5:1-4.
13. Giri AV, Anandkumar N, Muthukumaran G, Pennathur G. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol 2004;4:11. [DOI:10.1186/1471-2180-4-11]
14. Shahitha S, Poornima K. Enhanced production of prodigiosin production in serratia marcescens. J Appl Pharm Sci 2012;02:138-40. [DOI:10.7324/JAPS.2012.2823]
15. Nazemi A, Mehrabi M, Nasrollahi A. Isolation and molecular identification of pigment producing microorganisms and acute toxicity of pigments. Journal Of Microbial Biotechnology 2011;3:19-28.
16. Asgari A, Safari N, Zare D. Purification and Partial Identification of Carotenoid Pigment Haloarcula IRU1.sp A highly salty ark isolated from Lake Urmia. Journal Of Cellular And Molecular Research (Iranian Journal Of Biology) 2013;26:326-8.
17. Malik K, Tokas J, Anand RC. Characterization and Cytotoxicity Assay of Pigment Producing Microbes. Int J Curr Microbiol App Sci 2016;5:370-6. [DOI:10.20546/ijcmas.2016.506.042]
18. Basserisalehi M, Bahador N. Diagnostic Bacteriology. Navy Publication. 2012.
19. Andrews JM, Howe RA; BSAC Working Party on Susceptibility .Testing BSAC standardized disc susceptibility testing method (version 10). J Antimicrob Chemother 2011;66:2726-57. [DOI:10.1093/jac/dkr359]
20. American Diabetes Association. 2. Classification and diagnosis of diabetes. Diabetes Care 2016;1:13-22.
21. Oliveira P, Guelho D, Cardoso L, Vicente N, Martins D, Oliveira D, et al. Microbiological profile in diabetic foot infections: identification and susceptibility profile of bacteria isolated in 5 years in a Portuguese tertiary care hospital. Endocrine Abstracts 2016.
22. Do DC. The Role of Bacterial Biofilms in Chronic Infections; UC Riverside Electronic Theses and Dissertations. Schiller, Neal L: University of California 2014;93-6.
23. Yoon JH, Kang SJ, Lee JS, Oh TK. Brevundimonas terrae sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol 2006;56:2915-9. [DOI:10.1099/ijs.0.64253-0]
24. Wang K, Zhang L, Liu Y, Pan Y, Meng L, Xu T, et al. Kocuria dechangensis sp. nov., an actinobacterium isolated from saline and alkaline soils. Int J Syst Evol Microbiol 2015;65:3024-30. [DOI:10.1099/ijs.0.000372]
25. Singh H, Du J, Yang JE, Yin CS, Kook M, Yi TH. Brachybacterium horti sp. nov., isolated from garden soil. Int J Syst Evol Microbiol 201



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