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:: Volume 28, Issue 2 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(2): 104-116 Back to browse issues page
Role of extracted pigment from environmental isolated bacteria on antibiotic resistant isolated microorganisms from diabetic patients
Noushin Nahid 1, Nima Bahador * 2, Nematollah Razmi 3
1- MSc Student of Microbiology, Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Fars, Iran; Department of Microbiology, College of Science, Agriculture and Modern technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Associate Professor Microbiology, Department of Microbiology, College of Science, Agriculture and Modern technology Shiraz branch, Islamic Azad University, Shiraz, Iran , bahador@iaushiraz.ac.ir
3- Associate Professor Microbiology, Department of Microbiology, College of Science, Agriculture and Modern technology Shiraz branch, Islamic Azad University, Shiraz, Iran
Abstract:   (263 Views)
Background: Diabetes is one of the most important metabolic diseases in the world that can ultimately lead to ulceration and amputation of lower limbs. The aim of this study was pigment extraction from isolated environmental bacteria and their effect on antibiotic resistant bacteria cooperated on infected diabetic foot.
Materials and methods: In this project, dominant bacteria were identified using biochemical tests and antibiotic susceptibility was evaluated. Then, effect of different solvent was evaluated on the extraction of pigments using thin layer chromatography. Finally the affected bacteria were molecularly identified.
Results: The results obtained from this study indicated that the most common isolated bacteria were Escherichia coli and Staphylococcus aureus. The results from antibiogram tests indicated that most of the gram positive isolates had same results and the gram negative was sensitive to Meropenem and Imipenem. Among extracted pigments, 4 different pigment which is effective on antibiotic resistant bacteria according to molecular technique were Brachybacterium sp. IARI-ABL-35, Brachybacterium nesterenkovii strain DSM 9573, Brevundimonas sp. NeomS2D Kocuria sp. YIM 75764.
Conclusion: According to biologic properties of bacterial pigments, we could use them in the treatment of various diseases.
Keywords: Antibiotic susceptibility, Bacterial infection, Bacterial pigment, Diabetes mellitus, Thin-layer chromatography.
Full-Text [PDF 2343 kb]   (85 Downloads)    
Type of Study: Experimental | Subject: Microbiology
Received: 2017/05/17 | Accepted: 2017/09/3 | Published: 2018/06/19
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Nahid N, Bahador N, Razmi N. Role of extracted pigment from environmental isolated bacteria on antibiotic resistant isolated microorganisms from diabetic patients . MEDICAL SCIENCES. 2018; 28 (2) :104-116
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Volume 28, Issue 2 (winter 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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