:: Volume 28, Issue 1 (spring 2018) ::
MEDICAL SCIENCES 2018, 28(1): 37-43 Back to browse issues page
The expression studies of Bcl-xl gene subsequent effect of cholestasis and treatment by curcumin in hippocampus of male rats
Somayeh Baghbaderani 1, Mohammad Nasehi * 2, Mehrdad Hashemi 3
1- Master of Science, Department of Genetics, Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran
2- Associated Professor Department of Physiology, Institute for Cognitive Science Studies (ICSS), Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran , nasehi@iricss.org
3- Professor Department of Genetics, Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran
Abstract:   (793 Views)
Background: Cholestasis is a hepatic disease, which discomposes the secretion of bile and gives rise for the aggregation of bile compounds such as bile salts, in case of non-treatment, cholestasis finds the capability to influence on different organs such as heart and brain. Besides, the disease is also quite effective on the expression rate of apoptosis and mitochondrial biogenesis genes. Thus, we went through researching about the effects of cholestasis over the changes of expressions for genes run through apoptosis (BCL-XL) in hippocampus region of male rats. We also tried for fact-finding about the impact of curcumin drug, which holds treatment impact for chronic disease such as neoplastic, inflammatory, and nervous disorders over these genes expression in rats’ hippocampus.
Materials and methods: The animals were divided in 4 groups of BDL, BDL- Curcumin, Sham- Curcumin, and Control. Rats of BDL group experienced BDL surgery (closing bile tubes). Besides, of surgery, rats of BDL-Curcumin group, treated with curcumin. Animals of Sham-Curcumin just received surgery stress and treated with drug, while the animals of CONTROL just experienced surgery stress without any other interference. Then, we removed hippocampus from rats’ brains and after the RNA extraction and cDNA synthesis, we investigated genes expression measurement through Real Time PCR technique.
Results: Curcumin drug increased the rate of BCL-XL gene expression at the rats’ hippocampus.
Conclusion: The data indicated that curcumin can alter apoptosis-induced by cholestasis at the hippocampus region.
Keywords: Cholestasis, BCL-XL, Apotosis, Hippocampal, Curcumin.
Full-Text [PDF 387 kb]   (126 Downloads)    
Type of Study: Experimental | Subject: Genetic
Received: 2017/05/14 | Accepted: 2017/09/6 | Published: 2018/04/1
References
1. Elferink RO. Cholestasis. Gut 2003;52:ii42-8. [DOI:10.1136/gut.52.suppl_2.ii42]
2. Schmidt O, Pfanner N, Meisinger C. Mitochondrial protein import: from proteomics to functional mechanisms. Nat Rev Mol Cell Biol 2010;11:655-67. [DOI:10.1038/nrm2959]
3. Wang Z, Jiang H, Chen S, Du F, Wang X. The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways. Cell 2012;148:228-43. [DOI:10.1016/j.cell.2011.11.030]
4. Hirschfield GM, Heathcote EJ, Gershwin ME. Pathogenesis of cholestatic liver disease and therapeutic approaches. Gastroenterology 2010;139:1481-96. [DOI:10.1053/j.gastro.2010.09.004]
5. Trauner M. Molecular alterations of canalicular transport systems in experimental models of cholestasis: possible functional correlations. Yale J Biol Med 1997;70:365-78.
6. Kronsten V, Fitzpatrick E, Baker A. Management of cholestatic pruritus in paediatric patients with alagille syndrome: the King's College Hospital experience. J Pediatr Gastroenterol Nutr 2013;57:149-54. [DOI:10.1097/MPG.0b013e318297e384]
7. Paumgartner G. Medical treatment of cholestatic liver diseases: From pathobiology to pharmacological targets. World J Gastroenterol 2006;12:4445-51. [DOI:10.3748/wjg.v12.i28.4445]
8. Aggarwal BB, Sung B. Pharmacological basis for the role of curcumin in chronic diseases: an age-old spice with modern targets. Trends Pharmacol Sci 2009;30:85-94. [DOI:10.1016/j.tips.2008.11.002]
9. Singh S. From exotic spice to modern drug? Cell 2007;130:765-8. [DOI:10.1016/j.cell.2007.08.024]
10. Sharma RA, Gescher AJ, Steward WP. Curcumin: the story so far. Eur J Cancer 2005;41:1955-68. [DOI:10.1016/j.ejca.2005.05.009]
11. Aggarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res 2003;23:363-98.
12. Shishodia S, Sethi G, Aggarwal BB. Curcumin: getting back to the roots. Ann N Y Acad Sci 2005;1056:206-17. [DOI:10.1196/annals.1352.010]
13. Rivera-Espinoza Y, Muriel P. Pharmacological actions of curcumin in liver diseases or damage. Liver Int 2009;29:1457-66. [DOI:10.1111/j.1478-3231.2009.02086.x]
14. Ernst A, Alkass K, Bernard S, Salehpour M, Perl S, Tisdale J, et al. Neurogenesis in the striatum of the adult human brain. Cell 2014;156:1072-83. [DOI:10.1016/j.cell.2014.01.044]
15. Kempermann G, Song H, Gage FH. Neurogenesis in the Adult Hippocampus. Cold Spring Harb Perspect Biol 2015;7:a018812. [DOI:10.1101/cshperspect.a018812]
16. Reza Zarrindast M, Eslimi Esfahani D, Oryan S, Nasehi M, Torabi Nami M. Effects of dopamine receptor agonist and antagonists on cholestasis-induced anxiolytic-like behaviors in rats. Eur J Pharmacol 2013;702:25-31. [DOI:10.1016/j.ejphar.2013.01.023]
17. Wilhelm EA, Jesse CR, Roman SS, Nogueira CW, Savegnago L. Hepatoprotective effect of 3-alkynyl selenophene on acute liver injury induced by D-galactosamine and lipopolysaccharide. Exp Mol Pathol 2009;87:20-6. [DOI:10.1016/j.yexmp.2009.03.004]
18. Chan MM. Inhibition of tumor necrosis factor by curcumin, a phytochemical. Biochem Pharmacol 1995;49:1551-6. [DOI:10.1016/0006-2952(95)00171-U]
19. Kang BY, Chung SW, Chung W, Im S, Hwang SY, Kim TS. Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin. Eur J Pharmacol 1999;384:191-5. [DOI:10.1016/S0014-2999(99)00690-1]
20. Aggarwal BB. Prostate cancer and curcumin: add spice to your life. Cancer Biol Ther 2008;7:1436-40. [DOI:10.4161/cbt.7.9.6659]



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