:: Volume 27, Issue 3 (fall 2017) ::
MEDICAL SCIENCES 2017, 27(3): 178-186 Back to browse issues page
Investigating cytotoxic effect of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge on Hela cancer cells line
Anahita Behdarvand Shoushtar 1, Hossein Sazgar * 2, Abdollah Ghasemi Pirbaloti 3
1- M.Sc of Animal Physiology, Department of Biology, Faculty of basic Science
2- Ph.D of Animal Physiology, Department of Biology, Faculty of basic Science , hoseinsazgar@yahoo.com
3- Ph.D of Medicinal and Aromatic Plants, Department of Medicinal Plants
Abstract:   (1630 Views)
Background: Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was to investigate the cytotoxic effect of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge on Hela cancer cells line. Materials and methods: Hela cancer cells line and fibroblast normal cells line were cultured respectively in RPMI 1640 medium and DMEM medium with fetal bovine serum (FBS) and antibiotics. The cells exposed to different doses of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25 and 2.5 mg/ml) and were incubated for 24, 48 and 72 hours. After the incubation period, modified colorimetric MTT method was used to determine the cellular toxicity of the extract. Results: The results of the MTT test showed that hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge had dose-dependent and time-dependent anti-cancer effect on Hela cells line. The highest percentage of cell death was observed after 72 hours incubation and increase in extract concentration (p<0.05). Extract did not show significant cytotoxicity on fibroblast normal cells line. Conclusion: Hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge has cytotixic effect on Hela cells line. However this extract does not have any cytotoxic effect on fibroblast normal cells line. It seems that further researches can lead to detect effective compounds in the cancer treatment. Keywords: Kelussia odoratissma Mozaff, Satureja bachtiarica Bunge, MTT assay, Hela
Keywords: Kelussia odoratissma Mozaff, Satureja bachtiarica Bunge, MTT assay, Hela
Full-Text [PDF 393 kb]   (298 Downloads)    
Type of Study: Experimental | Subject: Physiology
Received: 2016/12/26 | Accepted: 2017/09/20 | Published: 2018/03/7


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Volume 27, Issue 3 (fall 2017) Back to browse issues page