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Alterations of gyrA in Enterobacteriaceae isolated of urinary tract infection resistance to quinolones antibiotics
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Atousa Monavari1   , Fatemeh Noorbakhsh 2, Roozbeh Yalfani3 |
1- MS student, Department of Microbiology, Biological Science College, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran
2- Department of Microbiology, Biological Science College, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran , niloofar_noorbakhsh@yahoo.com
3- Department of Nursing, School of medical Science, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran |
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Abstract: (4722 Views) |
| Background: Urinary tract infection is one of the most common infections, which if not treated, it can cause serious problems in patients. One of the ways to treat of this infection is antibiotic therapy. Nowaday, antibiotic resistance in microorganisms is a main problem for physicians and patients in the world. The aim of this study was to evaluate the genetic resistance to quinolones antibiotics in Enterobacteriaceae isolated in urine samples.
Materials and methods: 100 bacteria of Enterobacteriaceae family were isolated from suspected samples of urinary infection. Antibiotic susceptibility of isolated bacteria to quinolone antibiotics, including ciprofloxacin, nalidixic acid, norfloxacin, ofloxacin, levofloxacin and enrofloaxin, was performed by disc diffusion method according to standard guidelines (CLSI 2014). PCR was performed by specific primers of gyrA gene.
Results: Hundred bacteria were isolated of clinical urine sample including 60 E.coli, 32 Klebsiella, 3 Enterobacter, and 5 Proteus. Antibiotic resistance to ciprofloxacin were 36%, nalidixic acid 45%, norfloxacin 38%, ofloxacin 38%, levofloxacin 35% and enrofloaxin 39%. Totally, 36 bacteria were resist to all antibiotics, which 29 bacteria (80.55%) revealed mutation in gyrA gene.
Conclusion: This study revealed that Ecoli isolates carry a mutation in gyrA genes. This mutation has an important role in antibiotic resistance to quinolons.
Keywords: Antibiotics resistance, Quinolone, gyrA gene, Enterobacteracae, PCR. |
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| Keywords: Antibiotics resistance, Quinolone, gyrA gene, Enterobacteracae, PCR. |
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Full-Text [PDF 397 kb]
(1774 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Microbiology
Received: 2016/10/15 | Accepted: 2017/04/19 | Published: 2017/06/19
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