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:: Volume 27, Number 2 (summer 2017) ::
MEDICAL SCIENCES 2017, 27(2): 133-137 Back to browse issues page
Alterations of gyrA in Enterobacteriaceae isolated of urinary tract ‎infection resistance to quinolones antibiotics
Atousa Monavari1, Fatemeh Noorbakhsh *2, Roozbeh Yalfani3
1- MS student, Department of Microbiology, Biological Science College, Varamin-Pishva Branch,‎‏ ‏Islamic Azad ‎University, Varamin-Pishva, Iran
2- Assisstant Professor Department of Microbiology, Biological Science College, Varamin-Pishva Branch,‎‏ ‏Islamic Azad University, ‎Varamin-Pishva, Iran , niloofar_noorbakhsh@yahoo.com
3- Assisstant Professor Department of Nursing, School of medical Science, Varamin-Pishva Branch,‎‏ ‏Islamic Azad University, Varamin-‎Pishva,‎‏ ‏Iran
Abstract:   (284 Views)
Background: Urinary tract infection is one of the most common infections, which if not treated, it can ‎cause serious problems in patients. One of the ways to treat of this infection is antibiotic therapy. ‎Nowaday, antibiotic resistance in microorganisms is a main problem for physicians and patients in the ‎world. The aim of this study was to evaluate the genetic resistance to quinolones antibiotics in ‎Enterobacteriaceae isolated in urine samples. ‎ Materials and methods: 100 bacteria of Enterobacteriaceae family were isolated from suspected ‎samples of urinary infection. Antibiotic susceptibility of isolated bacteria to quinolone antibiotics, ‎including ciprofloxacin, nalidixic acid, norfloxacin, ofloxacin, levofloxacin and enrofloaxin, was ‎performed by disc diffusion method according to standard guidelines (CLSI 2014). PCR was ‎performed by specific primers of gyrA gene.‎ Results: Hundred bacteria were isolated of clinical urine sample including 60 E.coli, 32 Klebsiella, 3 ‎Enterobacter, and 5 Proteus. Antibiotic resistance to ciprofloxacin were 36%, nalidixic acid 45%, ‎norfloxacin 38%, ofloxacin 38%, levofloxacin 35% and enrofloaxin 39%. Totally, 36 bacteria were ‎resist to all antibiotics, which 29 bacteria (80.55%) revealed mutation in gyrA gene. ‎ Conclusion: This study revealed that Ecoli isolates carry a mutation in gyrA genes. This mutation has ‎an important role in antibiotic resistance to quinolons.‎ Keywords: Antibiotics resistance, Quinolone, gyrA gene, Enterobacteracae, PCR.‎
Keywords: Antibiotics resistance, Quinolone, gyrA gene, Enterobacteracae, PCR.‎
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Type of Study: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2016/10/15 | Accepted: 2017/04/19 | Published: 2017/06/19
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Monavari A, Noorbakhsh F, Yalfani R. Alterations of gyrA in Enterobacteriaceae isolated of urinary tract ‎infection resistance to quinolones antibiotics. MEDICAL SCIENCES. 2017; 27 (2) :133-137
URL: http://iau-tmuj.ir/article-1-1254-en.html
Volume 27, Number 2 (summer 2017) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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