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Detection of cmlA/tetR, bla PSE-1, bla TEM and sip B in the Salmonella strains by Multiplex-PCR method and their antibiotic resistance pattern
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Mohammad Reza Najafi1   , Mehdi Parviz 2, Kumarss Amini1 |
1- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Lecturer, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , dr_parviz89@yahoo.com |
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Abstract: (4765 Views) |
| Background: Gastroenteritis due to Salmonella is common in human and considered as a global dilemma of public health. This study was done to determine cmlA/tetR, bla PSE-1, bla TEM and sip B in the Salmonella strains by Multiplex-PCR method and their antibiotic susceptibility profile.
Materials and methods: In this cross-sectional study, 163 clinical samples were obtained from patients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for determination of these target genes by the specific oligonucleotides primers.
Results: Of 163 collected samples, 48(29.4%) Salmonella spp., were obtained, which 25(52.1%) were S. enteritidis, 14(29.2%) S. typhimurium and 9(18.7%) S. infantis. Antibiotic resistance analysis showed that the highest resistance rate were related to tetracycline (n: 27, 56.2%) and then streptomycin and chloramphenicol (n: 15, 31.2%). All isolates (n: 48, 100%) were susceptible to imipenem, amikacin, gentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of Salmonella spp., isolates carried cml/tetR and sipB genes, respectively.
Conclusion: According to our results, detection and genotyping of virulence genes and comparison with global ranging is a basic requirement in the control and prevention of salmonellosis in industrial purposes.
Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR |
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| Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR |
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Full-Text [PDF 288 kb]
(1179 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Microbiology
Received: 2016/11/27 | Accepted: 2017/04/19 | Published: 2017/06/19
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