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Isolation and identification of aflatoxin- producing Aspergillus flavus and Aspergillus parasiticus by PCR method from cattle feed in Shahr Ghods – Shahriar districts
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Shokoufeh Moradi1   , Azar Sabokbar 2, Ramin Hajikhani3 , Amir Bakhtiari4 , Somayeh Talebi5 |
1- MSc of Microbiology, Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran
2- Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran , sabokbar@kiau.ac.ir
3- Department of Biology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran
4- MSc of Genetics, Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran
5- PhD candidate of Microbiology, Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran |
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Abstract: (5995 Views) |
Background: Some of Aspergillus species are potential generators of aflatoxin that is a mycotoxin with carcinogenic and teratogenic effects. Animal feed contaminated with Aspergillus species produce aflatoxin, and transfer it to milk and dairy products that cause animal and human health problems. The aim of this study was to isolate any potential aflatoxinogen of animal feeds from farms area in the Shahr Ghods – Shahriar districts
Materials and methods: In this study, 394 cattle feed from 41dairy farming in Shahr Ghods – Shahriar districts in autumn, 2014, were randomly selected and they were tested for contamination with potentially aflatoxin-producing Aspergillus by PCR. Isolated Aspergillus from animal feed, by smear and culture, were examined by microscopic and macroscopic and then by molecular method. PCR method used in the presence of primers AFLR, Omt1, FLA1, Nor1, and Par1 to identify potential toxin generating Aspergillus.
Results: The results of study showed 42 cattle feed contaminated with 8 species Aspergillus flavus by PCR which could potentially produce aflatoxin.
Conclusion: The results showed that the dairy farming in Shahr Ghods – Shahriar districts were contaminated with Aspergillus flavus.
Keywords: Aflatoxin, Animal feed, PCR. |
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| Keywords: Aflatoxin, Animal feed, PCR. |
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Full-Text [PDF 274 kb]
(4806 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Mycology
Received: 2015/09/5 | Accepted: 2016/04/13 | Published: 2016/09/17
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