Surveying the effect of 100 kD protein of shark cartilage on erythroleukemic K562 cell line and normal lymphocytes
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Behnaz Riaz-Alhosseini , Mohammadhasan Zahir , Ali MOstafaie 1, Mehdi Mahdavi , Mehrdad Hashemi |
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Abstract: (19741 Views) |
Background: Shark cartilage is used in complementary medicine for the removal of tumor in cancer patients. However, scanty reports have addressed the effect of 100 KDa fraction on immunity system. The aim of the present study was to evaluate the effect of this fraction in human erythroleukemic K562 cell line and normal lymphocytes.
Materials and methods: Gel chromatography purification strategy was employed for purification of 100 KDa fraction from the shark cartilage. Erythroleukemic K562 cell line and normal lymphocytes were cultured in RPMI 1640 (Sigma), supplemented with 10% fetal calf serum, peniciline-streptomycin, L-glutamine and incubated at 37 ºC for 2 days. Normal mononuclear peripheral blood cells were collected by ficole. Cellular vital capacity was determined by MTT.
Results: During MTT, erythroleukemic K562 cell line revealed to have a meaningful suppression at 15g protein concentration when compared with controls (p<0.01). Conclusion: To our knowledge, it is the first study that demonstrates 100kD protein of shark cartilage to induce cell death in erythroleukemic K562 cell line in vitro. |
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Keywords: Shark cartilage, Cancer therapy, K562 cell line, MTT test, Lymphocyte. |
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Subject:
Epidemiology Received: 2006/09/6 | Published: 2007/06/15
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