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Association of interleukin-22 gene polymorphism (rs1179251) and colorectal cancer risk in the Iranian population
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Seyed Reza Mohebbi1   , Khatoon Karimi2 , Fatemeh Rostami3 , Seyed Mohammad Ebrahim Tahaei4 , Pedram Azimzadeh5 , Sara Romani2 , Hanie Mirtalebi6 , Mohammad Reza Zali 7 |
1- PhD of Medical Virology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- MSc of Microbiology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- MSc of Biochemistry, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- MSc of Virology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- MSc of Cellular and Molecular Biology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6- MSc of Cellular Biology and Embryology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7- Gastroenterologist, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , nnzali@hotmail.com |
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Abstract: (15908 Views) |
Background: Interleukin-22 (IL-22) is recognized as a key factor in chronic inflammatory disorders. Chronic inflammation is one of the major causes of CRC. Therefore polymorphisms occur in the inflammatory genes could modulate the levels of disease. We have investigated the association between single nucleotide polymorphism (SNP) in IL-22 gene (rs1179251) and CRC risk in the Iranian population.
Materials and Methods: This case- control study was done on 166 CRC patients and and 236 health control subjects. Genomic DNA is isolated from peripheral blood leukocytes by salting out method and was genotyped using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
Results: The frequencies of GG, GC, CC genotypes in case and control groups were 71.7%, 25.3%, 3.0% and 69.5%, 26.7%, 3.8%, respectively. No significant association was found between IL-22 gene polymorphism and risk of developing CRC (p=0.85).
Conclusion: Our data suggest that rs1179246 in IL-22 gene was not associated with the CRC susceptibility in the Iranian population. |
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| Keywords: Polymorphism, Interleukin-22, Colorectal cancer |
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Full-Text [PDF 199 kb]
(3105 Downloads)
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Semi-pilot: case-control |
Subject:
Molecular Biology
Received: 2014/01/22 | Accepted: 2014/01/22 | Published: 2014/01/22
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