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Tumor necrosis factor-α -308 G/A polymorphism in Iranian patients with chronic hepatitis C
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Shaghayeagh Baradaran Ghavami1   , Seyed Reza Mohebbi 2, Abbas Akhavan Sepahi3 , Hamed Naghoosi4 , Seyed Mohammad Ebrahim Tahaei5 , Pedram Azimzadeh6 , Sara Romani4 , Afsaneh Sharifian7 , Mohammad Reza Zali7 |
1- MSc of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
2- PhD of Medical Virology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , srmohebbi@gmail.com
3- PhD of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
4- MSc of Microbiology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- MSc of Medical Virology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6- MSc of Cell and Molecular Biology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7- Gastroenterologist, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
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Abstract: (10819 Views) |
Background: The impact of genetic changes like Single nucleotide polymorphisms on expression rate and function of cytokine is under study. The aim of the present study was to determine TNF-α cytokine gene polymorphism -308 G/A association with the susceptibility to hepatitis C (HCV) chronic infection.
Materials and Methods: In this case-control study, 152 HCV infected patients and 164 healthy controls were studied. Genotyping was carried out by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and the distribution of the TNF-α Gene -308 G/A polymorphism was compared in these groups. Tetra-primer ARMS-PCR employs two pairs of primers to amplify two alleles in one PCR reaction.
Results: The frequency of the TNF-α gene polymorphism at position -308 in HCV patients was GG (88.8%), GA (11.2%), AA (0%) and in healthy controls was GG(75%) ,GA (24%), AA (0%) (OR=2.626, 95%CI=1.419-4.860, P=0.002). There was a significant difference in genotype or allele frequency between HCV patients and control group (P<0.05).
Conclusion: Present study showed an association between TNF-α promoter polymorphism (-308) and hepatitis C in an Iranian population. |
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| Keywords: Genotype, Chronic Hepatitis C, Polymorphism, single nucleotide, Tumor Necrosis factor- alpha. |
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Full-Text [PDF 288 kb]
(4807 Downloads)
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Semi-pilot: case-control |
Subject:
Microbiology
Received: 2013/06/19 | Published: 2013/06/15
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