[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 4 (winter 2012) ::
MEDICAL SCIENCES 2012, 21(4): 238-243 Back to browse issues page
Cytotoxicity effect of ICD-85 (Venom – derived peptides) on promyelocytic cancer cell lines (HL-60)
Zahra Shahramyar * 1, Abbas Zare Mirakabadi 2, Hassan Morovati 3
1- MSc in Biochemistry, Razi Vaccine and Serum Research Institute, Karaj, Iran , zshahramyar@gmail.com
2- Associated Professor of Venomous Animals and Antivenin Production Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
3- MSc in Vaccine and Serum, Quality Control Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
Abstract:   (15040 Views)
Background: Our previous studies revealed an inhibitory effect of ICD-85 a venom derived peptide has been extracted by researchers of Razi vaccine and serum research institute, on breast cancer cell line (MDA-MB231). The present study was undertaken to evaluate the anti proliferative effect of HL60 cells.
Materials and methods: The effect of ICD-85 on proliferation of HL-60 cancer cells was determined using the MTT assay. A quantitative cytotoxicity assay based on the release of lactate dehydrogenase (LDH EC 1.1.1.27) was performed 24 h after exposure to various concentrations of ICD-85. The morphological changes of ICD-85 treated HL-60 cancer cells were observed under electron microscope.
Results: ICD-85 induced marked concentration inhibition of cancer cell proliferation with an IC50 value of 0.04µg/ml following 24 h incubation. Electron microscopic findings of ICD-85 treated cells showed nuclear material condensation, endoplasmic reticulum dilation, mitochondria swelling or degradation, increased cytoplasmic vacuoles, reduction or disappearance in cytoplasmic process and decreased nuclear/cytoplasmic ratio. LDH determination of cultured media of HL60 cells exposed to various concentration of ICD-85 revealed no significant changes in LDH levels compared with untreated cells.
Conclusion: The results of present study indicated that ICD-85 inhibited the cancer cell proliferation by inducing apoptosis rather than necrosis.
Keywords: Anticancer, Promyelocytic leukemic, ICD-85, HL-60, IC50, Venom, MTT assay, cytotoxicity
Full-Text [PDF 392 kb]   (2047 Downloads)    
Type of Study: Experimental | Subject: Cilinical Biochemistry
Received: 2012/02/14
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shahramyar Z, Zare Mirakabadi A, Morovati H. Cytotoxicity effect of ICD-85 (Venom – derived peptides) on promyelocytic cancer cell lines (HL-60). MEDICAL SCIENCES. 2012; 21 (4) :238-243
URL: http://iau-tmuj.ir/article-1-521-en.html


Volume 21, Issue 4 (winter 2012) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3781