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Frequency of TNFα-244GàA, TNFα-308GàA and TNFα-238GàA polymorphisms in healthy and malaria infected patients of Kerman and Hormozgan provinces
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Parisa Atef Vahid1   , Mohammad Reza Alivand2 , Feyzollah Hashemi Gorji1 , Mehrdad Hashemi1 , Mohammad Reza Noori Dalooi 3 |
1- Islamic Azad University, Tehran, Iran
2- Institute of Genetic Engineering and Biotechnology, Tehran, Iran
3- Tehran Medical University, Tehran, Iran , nooridaloii@sina.tums.ac.ir |
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Abstract: (19914 Views) |
Background: Tumor necrosis factor-alpha (TNFα) is one of the key cytokines that affects on pathology of microbial infections. It is suggested that genetic susceptibility to severe form of malaria is associated with TNFα promoter polymorphisms. The aim of present study was to investigate frequency of -308GàA, -244GàA and -238GàA polymorphism in 174 samples of Hormozgan and Kerman provinces.
Material and methods: In case- control study, we investigated the prevalence of TNFα-244GàA, TNFα-308GàA and TNFα-238GàA polymorphism in 174 (154 healthy and 20 patients) individuals from Kerman and Hormozgan provinces of Iran. DNA extraction performed, and then PCR-RFLP was used to detect polymorphisms in -308,-244 and -238 loci.
Results: In these three regions, 82.2% of healthy samples showed GG genotype, 14.26% AG and 0.19% AA. All samples of patients showed GG genotype in these three regions.
Conclusion: It seems that evolutionary effect of malaria causes increased genotypes which related with protection against malaria or reduction of risk of cerebral malaria and death, although, there was no association between frequency of these genotypes and protection against malaria. |
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| Keywords: Malaria, polymorphism, TNFα, PCR-RFLP |
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Full-Text [PDF 558 kb]
(3807 Downloads)
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Semi-pilot: case-control |
Subject:
Genetic
Received: 2011/07/6 | Published: 2011/06/15
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