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Evaluation of correlation of miR-141 expression changes with common mutations of K-Ras gene and its relationship with prognosis in tumor tissue of colorectal cancer in Iranian patients
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Melika Arkani1    , Ardeshir Hesampour Mahallati 2, Maliheh Entezari3 |
1- MSc, Department of Genetics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Biology, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran , a.hesanpour@gmail.com
3- Department of Genetics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran |
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Abstract: (687 Views) |
Background: In many human malignant tumors, the expression of micro RNA molecules is dysfunctional. The purpose of this study was to investigate the miR-141 marker as a possible prognostic marker in colorectal cancer in order to screen patients with K-Ras mutation.
Materials and methods: In this case- control study, 100 patients with colorectal cancer aged over 50 years were studied. The pathologist confirmed colorectal cancer among samples of paraffin-embedded colon tissue in the pathology department of Imam Hossein Hospital in Tehran. People who have codon 12 and 13 mutations in exon 2 of K-Ras gene were selected as case group and tumor samples of people without codon 12 and 13 mutation in exon 2 of K-Ras gene were selected as control group. Changes in miR-141 expression were investigated in both groups.
Results: The results showed that 20% of patients were positive for codon 12 mutation and 10% of patients had codon 13 mutation. miR-141 gene expression in K-Ras positive tumor samples was 1.5 times its expression level in K-Ras negative samples and this increase in expression was statistically significant.
Conclusion: The results of the present study showed that the frequency of common K-Ras gene mutations in the population of colorectal cancer patients under study is consistent with the average of the Iranian population. It seems that miR-141 can be used as a biomarker to help in the diagnosis and prognosis of colorectal cancer.
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| Keywords: Colorectal Cancer, K-Ras gene, miR-141 Marker, Real Time PCR, Electrophoresis |
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Full-Text [PDF 431 kb]
(253 Downloads)
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Semi-pilot: case-control |
Subject:
Genetic
Received: 2022/12/25 | Accepted: 2023/04/19 | Published: 2023/10/2
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Arkani M, Hesampour Mahallati A, Entezari M. Evaluation of correlation of miR-141 expression changes with common mutations of K-Ras gene and its relationship with prognosis in tumor tissue of colorectal cancer in Iranian patients. MEDICAL SCIENCES 2023; 33 (3) :230-239 URL: http://tmuj.iautmu.ac.ir/article-1-2088-en.html
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