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Simultaneous effects of curcumin and Fe3o4 nanoparticles on expression of Bad and Bclx genes in mitochondrial apoptosis in rats with testicular ischemia
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Shabnam Zarei Moradi1   , Seyed Abdolhamid Angaji2 , Mitra Salehi3 , Mehrdad Hashemi 4 |
1- Department of Genetics, faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
2- Department of Cell and Molecular Biology, Faculty of biosciences, Kharazmi university, Tehran, Iran
3- Department of Microbiology, faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
4- Department of Genetics, Faculty of Advanced Science and Technology, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran ---- Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mhashemi@iautmu.ac.ir |
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Abstract: (684 Views) |
Background and Aims: Testicular ischemia / reperfusion is one of the most serious urological emergencies that is generally identified in men, adolescents and infants. This causes damage to the testicles which, if misdiagnosed and treated improperly, can lead to infertility in men. In the present study, the effects of curcumin and Fe3O4 nanoparticles on the rate of apoptosis and expression of BAD, BCL-X genes were studied.
Materials and Methods: 48 adult rats aged 20 weeks and weighing 250 g in the control and ischemia / reperfusion (I / R) groups were included in the study. After induction of testicular torsion in the experimental group, both groups were treated with curcumin and iron nanoparticles at a dose of LD50 for 10 days and subcutaneously. Then the testes were removed and the survival and apoptosis of the cells were assessed by MTT and flow cytometry and the expression of BAD and BCLX genes was measured.
Results: Increased apoptosis was observed in testicular tissue cells of rats with ischemia / reperfusion injury. Exposure to iron nanoparticles increased BAD proapoptotic gene expression and decreased BCLX anti-apoptotic gene expression. Co-administration of curcumin and iron nanoparticles offset the adverse effects of nanoparticles. (P <0.05)
Discussion and Conclusion: It seems that co-administration of Fe3O4 nanoparticles with curcumin is effective in reducing the damage caused by testicular torsion due to its antioxidant properties and reduces necrosis. The synthesis of green nanoparticles can also be considered.
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| Keywords: BAD, BCLX, testicular torsion, gene expression, apoptosis, iron nanoparticles |
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Received: 2022/08/24 | Accepted: 2021/12/31 | Published: 2021/12/31
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