[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 19, Issue 2 (Summer 2009) ::
MEDICAL SCIENCES 2009, 19(2): 93-96 Back to browse issues page
Quantitative real-time PCR technique for rapid diagnosis of trisomy 21
Ahmad Reza Kamyab 1, Naser Masrouri , Mina Hayat-No Saied , Somayeh Jamali , Mehrdad Hashemi , Morteza Karimipour , Gholam Reza Javadi , Reza Mahdian
1- , kamyab10000@gmail.com
Abstract:   (23056 Views)
Background: Prenatal diagnosis and prevention of live-born children with Down syndrome is a principle priority of Iran’s ministry of health. The aim of this study was the rapid diagnosis of Down syndrome by quantitative real-time PCR technique as a new method for prenatal diagnosis.
Material and methods: In this experimental study, two milliliters of peripheral blood was obtained from each patient and normal control. Then genomic DNA was extracted using salting out method. DYRK1A2 gene as target gene and PMP22 gene as reference gene were analyzed by quantitative real-time PCR technique.
Results: DYRK1A2/PMP22 gene ratio was 1.68±0.13 and 1.00±0.09 in Down syndrome and normal samples, respectively (p<0.001), demonstrating 3 copies of target (DYRK1A2) gene in trisomy 21 syndrome and 2 copies in normal individuals.
Conclusion: DYRK1A2/PMP22 gene ratio is significantly higher in patients with Down syndrome compared with normal individuals. So, quantitative real-time PCR technique can be used as a sensitive, accurate and reliable technique for rapid diagnosis of trisomy 21 syndrome.
Keywords: Real-Time PCR technique, Down syndrome, rapid and prenatal diagnosis, gene dosage
Full-Text [PDF 152 kb]   (3467 Downloads)    
Subject: Epidemiology
Received: 2006/09/6 | Published: 2009/07/15
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Kamyab A R, Masrouri N, Hayat-No Saied M, Jamali S, Hashemi M, Karimipour M, et al . Quantitative real-time PCR technique for rapid diagnosis of trisomy 21 . MEDICAL SCIENCES 2009; 19 (2) :93-96
URL: http://tmuj.iautmu.ac.ir/article-1-196-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 2 (Summer 2009) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4645