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:: Volume 19, Issue 1 (Spring 2009) ::
MEDICAL SCIENCES 2009, 19(1): 41-48 Back to browse issues page
The effect of adult mouse lung extracts growth factors in differentiation of umbilical cord stem cells into blood cells in vitro condition
Nasim Hayati Roodbari 1, Kazem Parivar , Homa Mohseni Kouchesfahani , Parichehr Yaghmaie
1- , nasimhayati@yahoo.com
Abstract:   (24472 Views)
Background: Stem cells (SCs) have great therapeutic indication due to their potency of self-renewal, multilineage differentiation, feasibility and safety for donors. In this study, adult mouse lung extracts containing hematopoietic growth factors were administered to umbilical cord, and evaluated the differentiation of umbilical cord stem cells into erythroid and myeloid lineages.
Materials and methods: In this basic and practical research, SCs were isolated from umbilical cord by enzyme digestion and cultured in appropriate culture medium. Subjects were divided into four groups: Experimental groups 1 and 2 (E1 and E2) which were exposed to 50% and 70% concentration of lung extract for 7 days, respectively, sham (Sh) group which did not exposed to lung extract and cultured for 7 days, and control group (C). E1, E2 and Sh groups were incubated for 7 days. All groups were evaluated by alkaline phosphatase detection kit for stem cells. Then, blood cells count and hematopoietic growth factors were assessed. ANOVA was used for data analysis.
Results: There were significant changes in E2 groups as compared with Sh and C groups, so that E2 group cells were differentiated into erythroid and myeloid lineages.
Conclusion: Growth factors in lung extract could have stimulatory effects on umbilical cord stem cell differentiation into blood cells.
Keywords: Umbilical cord, Stem cell, Lung extract, Differentiation, Erythroid lineages, Myeloid lineages
Full-Text [PDF 426 kb]   (3361 Downloads)    
Subject: Epidemiology
Received: 2006/09/6 | Published: 2009/04/15
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Hayati Roodbari N, Parivar K, Mohseni Kouchesfahani H, Yaghmaie P. The effect of adult mouse lung extracts growth factors in differentiation of umbilical cord stem cells into blood cells in vitro condition. MEDICAL SCIENCES 2009; 19 (1) :41-48
URL: http://tmuj.iautmu.ac.ir/article-1-180-en.html


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Volume 19, Issue 1 (Spring 2009) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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