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:: Volume 28, Issue 3 (spring 2018) ::
MEDICAL SCIENCES 2018, 28(3): 220-229 Back to browse issues page
Streptokinase enzyme extraction from Streptococcus agalactiae isolated from mucous membrane and investigation of fibrinolytic effects and its toxicity on healthy cells Hu02
Ali Akbar Hatami 1 , Anosh Eghdami * 2
1- 1MSc, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , eghdami49@gmail.com
Abstract:   (335 Views)
Background: Streptokinase, the most well-known fibrinolytic drug, has long been used in the treatment of myocardial infarction. This enzyme is produced from beta-hemolytic bacteria. In this study, the Streptokinase enzyme was extracted from Streptococcus agalactia strain with ATCC12386, isolated from the throat.
Materials and methods: After cultivating the bacteria in the Blood agar medium and evaluating their aggressive activity, the bacteria were transferred to the BHI broth medium and after incubation at 37 ° C for 24 hours, centrifuge was carried out at 4 ° C for 10000 g for 30 minutes. The contents of the tubes were separated by 0.45 micron acetate cellulose filter and the filters were considered as crude enzymes until fibrinolysis activity and non-toxicity test were performed.
Results: Among the extracted enzymes, the enzymes of the shamrock 2, 6, 10, 13, 14, 29 and 27 had fibrinolytic activity higher than 30% and extraction enzyme from the standard bacteria had less fibrinolysis activity. The toxicity of the extracellular enzyme was lower than that of other samples in 24 hours and 48 hours.
Conclusion: Extracting enzymes have a fibrinolysis effect, and this effect is higher in the bacteria isolated from the throat than the standard bacteria. The toxicity of the enzymes varies with time and concentration. It decreases with decreasing toxicity and increases with increasing time. And it can be described as a new, low-risk, and natural remedy for the elimination of blood clot sound.
 
Keywords: Streptococcus agalactiae, Streptokinase, Fibrinolytic effect, Normal fibroblast cells.
Keywords: Streptococcus agalactiae, Streptokinase, Fibrinolytic effect, Normal fibroblast cells.
Full-Text [PDF 325 kb]   (104 Downloads)    
Type of Study: Experimental | Subject: Microbiology
Received: 2018/01/15 | Accepted: 2018/03/6 | Published: 2018/09/22
ENG-PDF [PDF 271 KB]  (29 Download)
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Hatami A A, Eghdami A. Streptokinase enzyme extraction from Streptococcus agalactiae isolated from mucous membrane and investigation of fibrinolytic effects and its toxicity on healthy cells Hu02 . MEDICAL SCIENCES. 2018; 28 (3) :220-229
URL: http://iau-tmuj.ir/article-1-1444-en.html


Volume 28, Issue 3 (spring 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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