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:: Volume 28, Issue 3 (spring 2018) ::
MEDICAL SCIENCES 2018, 28(3): 195-206 Back to browse issues page
Evaluating the differentiation potential of human chorion-derived mesenchymal stem cells into myoblast-like cells
Nikoo Bana , Faezeh Faghihi 1, Mansooreh Soleimani2 , Nasim Hayati Roodbari3
1- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran , Faghihi.f@iums.ac.ir
2- School of Medicine, Iran University of Medical Sciences, Tehran, Iran
3- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (3743 Views)
Background: To restore the lost cells of patients diagnosed with Muscular Degenerative diseases, stem cell therapy has recently proved to be an effective strategy. Chorion is an ethically approved reservoir which contains mesenchymal stem cells with self- renewal properties and multilineage differentiation capacity. Therefore, the aim of this study was to evaluate the myogenic differentiation potential of human chorion-derived mesenchymal stem cells (C-MSCs) for the first time.
Materials and methods: Chorion was digested by using 0.3% Collagenase type II. Cells were characterized by using flowcytometry and differentiated into osteoblast and adipoblast lineages. To induce myogenic differentiation, C-MSCs were cultured in DMEM/F12 and 2% FBS supplemented with 10μM of 5-azacytidine overnight. Afterwards, the medium was replaced with DMEM/F12 supplemented with 10% FBS for two weeks. Real-time PCR and immunocytochemistry were used to evaluate the expression of myogenic markers.
Results: The expression of CD90, CD73 and CD44 antigen and their ability to differentiation into osteoblast and adipoblast lineages were confirmed. Although the expression of CTNT and MYH6 reduced second week, Real-time PCR results revealed significant upregulation of Desmin, MYH6 and Cardiac TroponinT at the end of the first week (P<0.05). Slight upregulation of MYOD and GATA-4 transcripts at first and second week were observed. ICC staining approved the expression of Desmin, cTnT and α-MHC.  
Conclusion: The results showed that C-MSCs were potent to differentiate into myoblast- like cells.
 
Keywords: Human chorion, Mesenchymal stem cells, 5-azacytidine, Myogenic differentiation.
Keywords: Human chorion, Mesenchymal stem cells, 5-azacytidine, Myogenic differentiation.
Full-Text [PDF 613 kb]   (2762 Downloads)    
Semi-pilot: Experimental | Subject: Molecular Biology
Received: 2017/07/24 | Accepted: 2017/11/29 | Published: 2018/09/22
ENG-PDF [PDF 1175 KB]  (91 Download)
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Bana N, Faghihi F, soleimani M, Hayati Roodbari N. Evaluating the differentiation potential of human chorion-derived mesenchymal stem cells into myoblast-like cells . MEDICAL SCIENCES 2018; 28 (3) :195-206
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Volume 28, Issue 3 (spring 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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