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:: Volume 28, Issue 1 (spring 2018) ::
MEDICAL SCIENCES 2018, 28(1): 74-80 Back to browse issues page
Surveying drug resistance of Staphylococcus aureus strains resistant to the antibiotic vancomycin in some hospitals in Rasht
Elham Amiri * 1, Masoomeh Anvari 2
1- MSc, Islamic Azad University of Rasht, Department of Biology, Faculty of Basic Sciences, Rasht, Iran , Elham.amiri56@ yahoo.com
2- Associated Professor Islamic Azad University, Rasht Branch, Department of Biology, Faculty of Basic Sciences, Rasht, Iran
Abstract:   (485 Views)
Background: Staphylococcus aureus, as a most important cause of nosocomial and community-acquired infections, shows resistance to a wide range of antibiotics. The aim of this study was the phenotypic and molecular study of clinical isolates of vancomycin-resistant Staphylococcus aureus isolated from hospitals in Rasht in a six- month period (February 2015 to July 95).
Materials and methods: 217 clinical samples were collected from different wards of hospitals in Rasht. Staphylococcus aureus isolates were identified by biochemical tests. To determine microbial resistance of the strains to antibiotic vancomycin, phenotypic tests of disk diffusion (according to the CLSI), and minimum Inhibitory concentration in microdilution broth method were used. Also, the presence of VanA gene, encoding resistance to the antibiotic vancomycin in separated isolate was evaluated by PCR method.
Results: 67 Staphylococcus aureus were identified. In the test determining resistance to the antibiotic by disk diffusion method, the results in terms of resistance rate against antibiotics was as follow: 10.5% chloramphenicol, 25.37% gentamicin, 37.32% tetracyclin, 38.80% vancomycin, 44.7% oxacillin and 100% penicillin. In the microdilution broth, 22.4% of the samples showed resistance to vancomycin. In PCR, no band was observed for genes VanA.
Conclusion: It is recommended that in molecular studies, the presence of genes VanA and VanB is assessed; since the resistance could be related to the presence of VanB gene.
Keywords: Staphylococcus aureus, Vancomycin, Antibiotic resistance, VanA.
Full-Text [PDF 190 kb]   (89 Downloads)    
Type of Study: Survey/Cross Sectional/Descriptive | Subject: Molecular Biology
Received: 2017/05/14 | Accepted: 2017/07/11 | Published: 2018/04/1
1. Cupane L, Pugacova N, Berzina D, Cauce V, Gardovska D, Miklasevics E. Patients with Panton- Valentine leukocidin positive Staphylococcus aureus infections run an increased risk of longer hospitalization. Int J Mol Epidemiol Genet 2012;3:48-55.
2. Silva EC, Antas Md, Monteiro B Neto A, Rabelo MA, Melo FL, Maciel MA. Prevalence and risk factors for Staphylococcus aureus in health care workers at a university hospital of Recife-PE. Braz J Infect Dis 2008;12:504-8. [DOI:10.1590/S1413-86702008000600012]
3. Gu J, Xu W, Lei L, Huang J, Feng X, Sun C, et al. LysGH15, a novel bacteriophage lysin, protects a murine bacteremia model efficiently against lethal methicillin-resistant Staphylococcus aureus infection. J Clin Microbiol 2011;49:111-7. [DOI:10.1128/JCM.01144-10]
4. Kluytmans J, van Belkum A, Verbrugh H. Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms and associated risks. Clin Microbiol Rev 1997;10:505-20.
5. Woo P, Lau SK, Wong SS, Yuen KY.Staphylococcus aureus subcutaneous abscess complicating acupuncture need for implementation of proper infection control guidelines. New Microbiol 2003;26:169-74.
6. Chambers HF, DeLeo FR. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol 2009;7:629-41. [DOI:10.1038/nrmicro2200]
7. Kaplan SL, Hulten KG, Gonzalez BE, Hammerman WA, Lamberth L, Versalovic J, et al. Three-year surveillance of community-acquired Staphylococcus aureus infections in children. Clin Infect Dis 2005;40:178- 91. [DOI:10.1086/430312]
8. Noble WC, Virani Z, Cree RG. Co-transfer of Vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiol Lett 1992;93:195-8. [DOI:10.1111/j.1574-6968.1992.tb05089.x]
9. Deresinski S. Methicillin-resistant Staphylococcus aureus: an evolutionary, epidemiologic, and therapeutic odyssey. Clin Infect Dis 2005;40:562-73. [DOI:10.1086/427701]
10. Mohanasoundaram KM, Lalitha MK. Comparison of phenotypic versus genotypic methods in the detection of methicillin resistance in Staphylococcus aureus. Indian J Med Res 2008;127:78-84.
11. Méndez-Álvarez S, Pérez-Hernández X, Claverie-Martín F. Glycopeptide resistance in enterococci. Int Microbiol 2010;3:71-80.
12. Muneeri SS, Mobaiyen H, Mirzaie H. Study on Vancomycin-resistant staphylococcus aureus and identification of VanA gene in these strains isolated from Tabriz Shuhada Hospital using e-test and PCR methods. Life Sci J 2013;10:748-52.
13. Weinstock DM, Conlon M, Iovino C, Aubrey T, Gudiol C, Riedel E, et al. Colonization, bloodstream infection, and mortality caused by vancomycin-resistant enterococcus early after allogeneic hematopoietic stem cell transplant. Biol Blood Marrow Transplant 2007;13:615-21. [DOI:10.1016/j.bbmt.2007.01.078]
14. Chang S, Sievert DM, Hageman JC, Boulton ML, Tenover FC, Downes FP, et al. Infection with vancomycinresistant Staphylococcus aureus containing the vanA resistance gene. N Engl J Med 2003;348:1342-7. [DOI:10.1056/NEJMoa025025]
15. Dutka-Malen S, Molinas C, Arthur M, Courvalin P. The VanA glycopeptide resistance protein is related to D-alanyl-D-alanine ligase cell wall biosynthesis enzymes. Mol Gen Genet 1990;224:364-72. [DOI:10.1007/BF00262430]
16. Moza B, Varma AK, Buonpane RA, Zhu P, Herfst CA, Nicholson MJ, et al. Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1. EMBO J 2007;26:1187-97. [DOI:10.1038/sj.emboj.7601531]
17. Mohajeri P, Izadi B, Rezaei M, Falahi B, Moradi Z, Zare ME. Study of nasal carriage Methicillin resistant Staphylococcus aureus in hemodialysis patients in Kermanshah. J Kermanshah Univ Med Sci 2012;15:485-92.
18. Saha B, Singh AK, Ghosh A, Bal M. Identification and Characterization of a vancomycin resistant staphylococcus aureus isolated from Kolkata (South Asia). J Med Microbiol 2008;57:79-2. [DOI:10.1099/jmm.0.47144-0]
19. Safdari H, Sadeghian A. Susceptibility of Staphylococcus aureus, the most common antibiotics Ghaem Hospital in Mashhad in year 1390. 1390;1:43-6.
20. Ahmadishoar SH, Nahaei MR, Amirmozafari N. Sensitivity of strains isolated from clinical specimens against vancomycin using E-test in tabriz. TUOMS 2008;30:17-23.
21. Nicoletti G, Schito G, Fadda G, Boros S, Nicolosi D, Marchese A, et al. Bactrial isolates from sever infections and their antibiotic susceptibility patterns in Italy: a among methicillin resistant Staphylococcus aureus isolates from intensive care units of Nation wide study in the hospital setting. J Chemother 2006;18:589-602. [DOI:10.1179/joc.2006.18.6.589]
22. Thati V, Shivannavar CT, Gaddad SM. Vancomycin resistance tertiary care hospitals in Hyderabad. Indian J Med Res 2011;134:704-8. [DOI:10.4103/0971-5916.91001]
23. Farhadian A, Behzadian Nejad Q, Najar Peerayeh SH, Rahbar M, Vaziri F. etermination of vancomycin and methicillin Resistance in clinical Isolates of staphylococcus aureus in Iranian Hospitals. Br Microbiol Res J 2008;4:454-61. [DOI:10.9734/BMRJ/2014/4836]
24. Hiramatsu K, Suzuki E,Takayama H, Katayama Y, Yokota T. Role ofpenicillinase plasmids in310the stability of the mecA gene in methicillinresistant Staphylococcus aureus. Antimicrob Agents Chemother 1990;34:600-4. [DOI:10.1128/AAC.34.4.600]
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Amiri E, Anvari M. Surveying drug resistance of Staphylococcus aureus strains resistant to the antibiotic vancomycin in some hospitals in Rasht. MEDICAL SCIENCES. 2018; 28 (1) :74-80
URL: http://iau-tmuj.ir/article-1-1376-en.html

Volume 28, Issue 1 (spring 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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