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:: Volume 28, Issue 1 (spring 2018) ::
MEDICAL SCIENCES 2018, 28(1): 44-49 Back to browse issues page
Effect of vitamin C on liver injury after taking methotrexate in rats
Behzad Jamali * 1, Sajjad Jamali 2, Nasser Shokri Kalehsar 3
1- Young Researchers and Elite club , Tabriz Branch, Islamic Azad University, Tabriz, Iran , behzadjamali100@yahoo.com
2- Young Researchers and Elite club , Tabriz Branch, Islamic Azad University, Tabriz, Iran
3- Department of Biochemistry, Ardabil branch, Islamic Azad University, Atdabil, Iran
Abstract:   (778 Views)
Background: Methotrexate is a folic acid antagonist which is widely used as a cytotoxic chemotherapeutic agent for leukemia and other malignancies. It causes structural and functional damages in liver. The aim of this study was to determine the effects of vitamin C on the liver enzymes, including alanine amino transferase (ALT) and spartate amino transferase (AST), and also liver tissue damages induces bytaking methotrexate.
Materials and methods: In this study, 30 rats were divided into three groups. Group 1 (control group) received normal saline (0.9%), intraperitoneally, for 10 consecutive days. Group 2 were given methotrexate (20mg/kg) intraperitoneally for 10 consecutive days. Group 3 received methotrexate with vitamin C (25mg/kg) intraperitoneally for 10 consecutive days. After 10 days, all animals were anaesthetized and blood serum were collected for biochemical analysis. After cervical dislocation, the liver tissue samples were taken in order to histological evaluation by light microscopy.
Results: Serum levels of AST and ALT were significantly lower in group of methotrexate and vitamin C compared to methotrexate group (p<0.05). The amount of liver tissue damages in group received methotrexate and vitamin C was lower than the group received only methotrexate (P<0.05).
Conclusion: The results of this study show that vitamin C may have a beneficial role in preventing liver damage caused by methotrexate and thus it could be considered potential role in clinical applications.
Keywords: Ascorbic acid, Liver, Damage, Methotrexate, Rat
Full-Text [PDF 320 kb]   (186 Downloads)    
Type of Study: Experimental | Subject: Labratory Sciences
Received: 2017/05/17 | Accepted: 2017/07/25 | Published: 2018/04/1
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Jamali B, Jamali S, Shokri Kalehsar N. Effect of vitamin C on liver injury after taking methotrexate in rats. MEDICAL SCIENCES. 2018; 28 (1) :44-49
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Volume 28, Issue 1 (spring 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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