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:: Volume 27, Number 2 (summer 2017) ::
MEDICAL SCIENCES 2017, 27(2): 119-125 Back to browse issues page
Detection of ‎‌‎ cmlA/tetR, ‎‌bla PSE-1, bla TEM and sip B in the ‎Salmonella strains by Multiplex-PCR method and their antibiotic ‎resistance pattern
Mohammad Reza ‎ Najafi‎1, Mehdi Parviz *2, Kumarss‎ Amini‎3
1- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- ‎Lecturer, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, ‎Saveh, Iran , dr_parviz89@yahoo.com
3- Assistant Professor‎ Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
Abstract:   (649 Views)
Background: Gastroenteritis due to Salmonella is common in human and considered as a global ‎dilemma of public health. This study was done to determine cmlA/tetR, ‎‌bla PSE-1, bla TEM and sip B ‎in the Salmonella strains by Multiplex-PCR method and their antibiotic susceptibility profile. ‎ Materials and methods: In this cross-sectional study, 163 clinical samples were obtained from ‎patients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined ‎using the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for ‎determination of these target genes by the specific oligonucleotides primers. ‎ Results: Of 163 collected samples, 48(29.4%) Salmonella spp., were obtained, which 25(52.1%) were ‎S. enteritidis, 14(29.2%) S. typhimurium and 9(18.7%) S. infantis. Antibiotic resistance analysis showed ‎that the highest resistance rate were related to tetracycline (n: 27, 56.2%) and then streptomycin and ‎chloramphenicol (n: 15, 31.2%). All isolates (n: 48, 100%) were susceptible to imipenem, amikacin, ‎gentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of ‎Salmonella spp., isolates carried cml/tetR and sipB genes, respectively. ‎ Conclusion: According to our results, detection and genotyping of virulence genes and comparison ‎with global ranging is a basic requirement in the control and prevention of salmonellosis in industrial ‎purposes.‎ Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR
Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR
Full-Text [PDF 288 kb]   (186 Downloads)    
Type of Study: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2016/11/27 | Accepted: 2017/04/19 | Published: 2017/06/19
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Najafi‎ M R ‎, Parviz M, Amini‎ K. Detection of ‎‌‎ cmlA/tetR, ‎‌bla PSE-1, bla TEM and sip B in the ‎Salmonella strains by Multiplex-PCR method and their antibiotic ‎resistance pattern. MEDICAL SCIENCES. 2017; 27 (2) :119-125
URL: http://iau-tmuj.ir/article-1-1252-en.html
Volume 27, Number 2 (summer 2017) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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