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:: Volume 26, Issue 2 (summer 2016) ::
MEDICAL SCIENCES 2016, 26(2): 76-81 Back to browse issues page
Evaluation of ESBL genes of blaTEM, blaSHV, blaoxa and aminoglycoside gene of aadA in Escherichia coli strains isolated in Tehran and determination of antimicrobial resistance
Shima Moeeni1 , Kumarss Amini 2
1- MSc, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran
2- Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran , dr_kumarss_amini@yahoo.com
Abstract:   (5446 Views)

Background: Nowadays, the widespread use of broad-spectrum beta-lactam antibiotics has increased antibiotic resistance around the world. The aim of this study was to assess and identify genes blaTEM, blaSHV, blaoxa and blaaada in clinical isolates E.coli by Multiplex- PCR methods.

Materials and methods: In this descriptive study, 150 stool samples from different treatment centers in Tehran were randomly gathered; and biochemical tests detected 55 E. coli. Antimicrobial susceptibility testing was performed by disk diffusion method according to CLSI. Multiple PCR assay was used to identify the genes β-lactamase. Data were analyzed by descriptive statistical analysis using SPSS version 19.

Results: Highest sensitivity was observed to cefpodoxime and trimethoprim (90.9% and 58.1% respectively) and the maximum resistance was reported to imipenem and ciprofloxacin (76.3% and 100% respectively). PCR results showed bla oxa gene in 40 isolates (72.7%), aadA gene in 15 samples (27.2%) and concurrent  blaoxa/ aadA genes in 12 isloates (21.8%). blaTEM and blaSHV genes were not identified in samples.

Conclusion: Antibiotic resistance to E.coli in children with diarrhea is indicated the indiscriminate use of antibiotics. Monitoring and control of drug-resistant intestinal bacteria play a major role in preventing the creation of resistant strains.

Keywords: Escherichia coli, Extended specterum β- lactamases, Multiplex-PCR.

Keywords: Escherichia coli, Extended specterum β- lactamases, Multiplex-PCR
Full-Text [PDF 228 kb]   (2560 Downloads)    
Semi-pilot: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2015/10/26 | Accepted: 2016/01/9 | Published: 2016/06/28
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Moeeni S, Amini K. Evaluation of ESBL genes of blaTEM, blaSHV, blaoxa and aminoglycoside gene of aadA in Escherichia coli strains isolated in Tehran and determination of antimicrobial resistance. MEDICAL SCIENCES 2016; 26 (2) :76-81
URL: http://tmuj.iautmu.ac.ir/article-1-1091-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 26, Issue 2 (summer 2016) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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